Generate a calibration curve to correlate the absorbance to the sucrose concentration. First, take the absorbance (OD) of Blank and make it zero. Heat the mixture at 90º C for 5-15 minutes to develop the red-brown color. PREPARATION. All monosaccaride and some disaccaride are reducing sugars v v … Thamara C. Coutinho, João O. D. Malafatti, Elaine C. Paris, Paulo W. Tardioli, Cristiane S. Farinas. Pipette out standard sugar solution in the range of 0 to 3 mL in different test tubes and make up the volume of all test tubes to 3 mL with distilled water concentrations ranging from 0 to 750 mg. Add 1 mL DNS reagent to all the test tubes and mix plug the test tube with cotton or marble and keep the test tube in a boiling water bath for 5 minute. Analar. After cooling to room temperature in a cold water bath, record the absorbance with a spectrophotometer at 540nm. This starch solution does not keep well and should be made up fresh on the required day. Add to a small amount of cold water in a beaker and make a slurry. Add 3 ml of DNS reagent to 3 ml of glucose sample in a lightly capped test tube. 12 PrepMan Ultra Sample Preparation Reagent Protocol About the PrepMan Ultra Sample Preparation Reagent Purpose of PrepMan Ultra Sample Preparation Reagent PrepMan® Ultra Sample Preparation Reagent provides a simple way to prepare DNA from a wide range of sample types including: † Processed foods and their ingredients † Bacteria † Fungi Make dilutions of glucose standards; Add 3 ml of DNSA reagent to all the eight test tubes. The DNSA test can detect concentrations of glucose between 0.5 mM (0.09% glucose w/v) and 40 mM (0.72% glucose w/v). Discussions The DNS method can be applied twice to measure the individual concentrations of a mixture of glucose and sucrose. Keep in boiling water bath for 15 minutes. (To avoid the loss of liquid due to evaporation, cover the test tube with a piece of paraffin film if a plain test tube is used.) 7.4.3.1 Use Glucose (HK) Assay Reagent, Prod. 7.4.3 Glucose (HK) Determination Vial. Dilute to a final volume of 100cm 3 with water. 4H 2 O) Add 20cm 3 of 2N NaOH. This article is cited by 15145 publications. Weigh out 2 g starch powder. Sucrose: (10%) No. Prepare arsenomolybdate reagent in three steps: Dissolve 25 g ammonium molybdate in 400 mL water and add 25 mL concentrated sulfuric acid and mix. The colour of the reagent changes from yellow to orange or red, depending upon the concentration of reducing sugar present. DNS method The DNS method for estimating the concentration of reducing sugars in a sample Reducing sugars contain free carbonyl group, have the property to reduce many of the reagents. Dissolve contents per label instructions. Dissolve 3 g sodium arsenate heptahydrate in 25 mL water. Mix and heat gently to make a uniform suspension. 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