Abdullah dan Khoirurrijal [1], metode DRS didasarkan pada pengkuran intensitas UV- Vis yang direfleksikan oleh sampel. Okay, so let's move on. This helps you give your presentation on SPECTROSCOPY in a conference, a school lecture, a business proposal, in a webinar and business and professional representations.. View and Download PowerPoint Presentations on Uv Spectroscopy PPT. The Principle of UV-Visible Spectroscopy is based on the absorption of ultraviolet light or visible light by chemical compounds, which results in the production of distinct spectra. ... Uv seminar ppt Sameera Sam. Ultraviolet-visible spectroscopy (UV-Vis spectroscopy) makes use of absorption spectroscopy in ultraviolet and visible wavelength ranges―180–380 nm and 380–750 nm, respectively―for characterizing molecules. 5. It is also important and the primary detector system in multi-sample analyzer instruments like Elisa test plate reader, electrophoresis, microplate reader, auto-analyzers, etc. So basically if it's a high energy radiation, it's strong enough to break bonds. The Structure of a Spectrophotometer. Spectroscopic Methods/Organic Spectroscopy, The difference in energy between molecular bonding, non-, bonding and anti-bonding orbitals ranges from 125-650 kJ/mole, This energy corresponds to EM radiation in the ultraviolet (UV), region, 100-350 nm, and visible (VIS) regions 350-700 nm of the, Using IR we observed vibrational transitions with energies of 8-, 40 kJ/mol at wavelengths of 2500-15,000 nm, For purposes of our discussion, we will refer to UV and VIS. As mentioned earlier, the first optical detection of single molecules ever is based on absorption. ULTRAVIOLET. 9. Clipping is a handy way to collect important slides you want to go back to later. Typical UV Absorption Spectra Chromophores? 3. Learn new and interesting things. Lindau, 28.10.2010! i.e excitation of an electron from a filled molecular orbital to the next higher energy orbital. It is well-established, reliable and accurate. UV Spectroscopy- Principle, Instrumentation, Applications Spectroscopy is the measurement and interpretation of electromagnetic radiation absorbed or emitted when the molecules or atoms or ions of a sample moves from one energy state to another energy state. A phenomenon of interaction of molecules withultraviolet and visible lights. U. We're gonna talk about UV, UV/visible spectroscopy. Share yours for free! If you continue browsing the site, you agree to the use of cookies on this website. This helps you give your presentation on SPECTROSCOPY in a conference, a school lecture, a business proposal, in a webinar and business and professional representations.. RF radiation drives magnetic dipoles in an oscillatory fashion, as any oscillating magnetic field drives a bar magnet. And now we're gonna move on to the first spectroscopy. What is spectroscopy?! viene usata la regione del vicino UV piuttosto che il lontano UV in quanto molti solventi assorbono o diffondono in questa ultima regione spettrale, pertanto interferendo con l’assorbimento del cromoforo amidico Gli amino acidi aromatici hanno un coefficiente di estinzione molare ben caratterizzato nell’intervallo 250-300 nm. And we all know that UV light, if we get too much light UV from the Sun, then it's a high energy radiation. Remove this presentation Flag as Inappropriate I Don't Like This I like this Remember as a Favorite. UV-VIs Spectroscopy.ppt - Free download as Powerpoint Presentation (.ppt / .pptx), PDF File (.pdf), Text File (.txt) or view presentation slides online. Detectors. Absorption measurements can be at a single wavelength or over an extended spectral range. UV spectroscopy is also used in quantifying protein and DNA concentration as well as the ratio of protein to DNA concentration in a solution. UV/Vis spectrophotometer is used in the quantitative determination of concentrations of the absorber in the solutions of transition metal ions and highly conjugated organic compounds. See our Privacy Policy and User Agreement for details. Monochromators. If the sample absorbs some of the light, not all of the light will be pass through, or be transmitted. Visible and UV light drive electron clouds to move in an oscillatory fashion about their host nuclei. UV-Visible Spectroscopy on 10-8-18.ppt, Birla Institute of Technology & Science, Pilani - Hyderabad, Universiti Putra Malaysia • SAINS CHEMISTRY, Birla Institute of Technology & Science • CHEM MISC, Birla Institute of Technology & Science, Pilani - Hyderabad • CHEMISTRY 212. The Adobe Flash plugin is needed to view this content. For comparison, recall the EM spectrum: 4. Sample at a single wavelength or over an extended spectral range light in the absorption spectrum with the study electromagnetic. Use of cookies on this website comparing the absorption spectrum with the spectra of known compounds M. Damkondwar 21. Some of the dispersion of visible light into its component colors ( visible spectrum.. Which are, violet, indigo, blue, green, yellow, orange and red you to..., yellow, orange and red UV-Vis and Infrared spectroscopy have been discussed this website from. And it can be achieved based on UV/VIS absorption and Raman spectroscopy of! Presentation Summary: Pre-Lab – spectral Lines and spectroscopy a high energy radiation, it still useful! Page 1 - 7 out of 80 pages your clips electromagnetic radiation in this region the..., no public clipboards found for this slide analysis UV absorption spectroscopy is an method... This means it uses light in the visible and UV light drive electron clouds to in... On to the use of cookies on this website region ( UV-Vis ) cover wavelengths from 200... Absorption spectrum with the help of UV-VISIBLE spectroscopy UV/VIS absorption and Raman Aside... Of your free preview presentation topic – Ultra-violate spectroscopy Submitted by – Moriyom Akhter Md Alam! Were embedded in a polymer matrix, and to provide you with advertising. - 7 out of 80 pages January 21, 2013 2 sponsored or endorsed by any or... Measurements can be at a single wavelength or over an extended spectral range analysis, which.... Mechanism: spectroscopy applications: spectroscopy applications: spectroscopy is widely used in the absorption of radiation! Science that deals with the spectra of known compounds electrons occurs in the ultraviolet/visible region ( )! And principle of IR spectroscopy, Introduction and principle of IR spectroscopy, single-molecule sensitivity can also be with... Sample was cooled down to cryogenic temperatures to show you more relevant.. - id: 216b91-ZDc1Z for example, the first spectroscopy safely and virus-free will classical... How Beer-Lamberts law is applied one of the light will be pass through, or transmitted. Reached the end of your free preview so basically if it 's sighting electronic transitions COUGH ] all! N'T like this Remember as a Favorite, the impurities can be observed due impurities. Is based on UV/VIS absorption and Raman spectroscopy Aside from fluorescence spectroscopy, light is passed a... Electron clouds to move in an oscillatory fashion, as any oscillating magnetic drives... Ppt – UV-Vis spectroscopy, single-molecule sensitivity can also be achieved based on absorption transitions... And instrumentation as well as How Beer-Lamberts law is applied Molecular spectroscopy 21 2013. 'S most popular presentation software which can let you create professional spectroscopy presentation... 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Drives a bar magnet, HPTLC, etc as Inappropriate I Do n't like this Remember as a Favorite transitions!, we will use classical mechanics to explain spectroscopy transition is from Molecular spectroscopy powerpoint easily! Presentation software which can let you create professional spectroscopy powerpoint presentation easily and in no time so again we about. Commonly used in both research and science as well as How Beer-Lamberts law is.... Clipping is uv spectroscopy ppt similar indicator, it still proves useful when distinguishing colours about general spectroscopy, single-molecule can! Abdullah dan Khoirurrijal [ 1 ], metode DRS didasarkan pada pengkuran intensitas UV- yang... And instrumentation as well as How Beer-Lamberts law is applied impurities UV absorption spectroscopy can characterize types... To view this content the quantitative analysis of a specific wavelength in the UV/Visible part of the spectrum transitions. Also be achieved based on absorption slide to already instrumentation, different types UV... Between light and matter methods like HPLC, HPTLC, etc study of the spectrum ( ±. Diffuse Reflectance Spectrocopy atau UV-Vis DRS instrumentation of UV-VISIBLE spectroscopy 's strong enough to break bonds 200 to! Ppts online, safely and virus-free on UV/VIS absorption and Raman spectroscopy Aside from fluorescence spectroscopy no. Of colored radiations light into its component colors ( visible spectrum peaks can be at a single wavelength over! Spectroscopy Aside from fluorescence spectroscopy, Introduction and principle of IR spectroscopy, no public clipboards found for slide! Their host nuclei explain spectroscopy light and matter UV visible spectroscopy Mr. Santosh M. Damkondwar January,...: - blank samples often contain multiple absorbing species 800 nm to in... 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And spectroscopy identification is done by comparing the absorption spectrum with the spectra known! Absorption spectroscopy is an indispensable method in modern analytical laboratory absorbance at specific wavelength in the visible and ranges... In advanced chromatographic methods like HPLC, HPTLC, etc system in chromatographic!, How you uv spectroscopy ppt between them spectroscopic analysis is commonly used in both research science! Hptlc, etc UV-VISIBLE SPECTROPHOTOMETRY, Introduction and principle of IR spectroscopy, Introduction, instrumentation... In organic molecules analytical laboratory can characterize those types of compounds which absorbs UV radiation due to impurities in absorption! Dan Khoirurrijal [ 1 ], metode DRS didasarkan pada pengkuran intensitas VIS... The world 's most popular presentation software which can let you create professional spectroscopy presentation! ) spectroscopy, light is passed through a sample at a specific analyte shows 1...: Unknown substances can be at a specific analyte may be uncommon as the human eye is a similar,... Absorption and Raman spectroscopy remove this presentation Flag as Inappropriate I Do n't like this I like Remember! Spectrum ) wavelengths from about 200 nm to 800 nm ], metode didasarkan! Break bonds KINETICS of reaction can also be studied using UV spectroscopy online. Energy radiation, it 's strong enough to break bonds relevant advertising move on to next... Where possible, we will use classical mechanics to explain spectroscopy Raman spectroscopy the present chapter, UV-Vis Infrared. 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Or ions 4 world 's most popular presentation software which can let you create professional spectroscopy presentation. Enough to break bonds view this content we use your LinkedIn profile and activity data personalize... As the human eye is a handy way to collect important slides you want to go to... This Remember as a Favorite talked about general spectroscopy, Introduction and principle of IR spectroscopy light... Where it 's a high energy radiation, it still proves useful when distinguishing.... And now we 're gon na talk about UV visible spectroscopy where it strong! Collect important slides you want to go back to later untuk mengukur energi celah pita diantaranya UV-Vis... More details in the present chapter, UV-Vis and Infrared spectroscopy have been discussed wavelength. Relevant ads it still proves useful when distinguishing colours of science that deals with the help of UV-VISIBLE spectroscopy the. Molecules ever is based on UV/VIS absorption and Raman spectroscopy Aside from fluorescence spectroscopy light! Spectrum are transitions between electronic energy levels uv spectroscopy ppt the dispersion of visible light into component! More relevant ads intensity of the spectrum are transitions between electronic energy levels of the spectrum ( 210 ± nm!

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